可用于荧光强度,时间分辨荧光,紫外可见吸收光,荧光偏振,化学发光和生物发光的检测
POLARstar OPTIMA - Technical Specifications
Measurement Principles
Fluorescence Polarization
Fluorescence Intensity
Time-Resolved Fluorescence
Absorbance
Luminescence
Light Source
High-energy xenon flash lamp
Detector
Side window, current type PMT
Filters
Two filter wheels for 8 filters each
Gain Control
Automatic Gain adjustment
Fluorescence Polarization
<5 mP standard deviation at 1 nM fluorescein
Fluorescence Intensity
Limit of detection: 0.9 fmol / well fluorescein
calculated according to the IUPAC standard: 3(SDblank)/slope
Time-Resolved Fluorescence
Limit of detection: 50 amol/well europium
calculated according to the IUPAC standard: 3(SDblank)/slope
Luminescence
Limit of detection: <34 amol/well ATP
calculated according to the IUPAC standard: 3(SDblank)/slope
Maximum counts per second: 2,000,000
DLR certified
Absorbance
Dynamic range: 0.000-3.000 OD
Reproducibility: 0.010 OD for 0-2 OD range
Microplate Formats
Up to 384
Fluorescence intensity and time-resolved fluorescence up to 1536
Reading Time
Dependent on test definition
shortest reading time: 15 s for 96-well plate, 30 s for 384-well plate and 60 s for 1536-well plate
Incubation
Incubation range from ambient +5°C to 45°C (option: 60°C)
Temperature stability 0.2°C
Temperature gradient < 0.5°C
Reagent Injectors
Up to 2 built-in syringe pumps with direct measurement access.
Variable injection speed
Individual volumes for each well
Four injection events per well
Reagent back flushing
Shaking
Orbital or linear shaking, user definable time and amplitude
Dimensions
Width 44 cm, length 48 cm and height 27 cm. Weight 28 kg
Stacker
For automatic plate handling, a Stacker is available with magazines for up to 25 plates (6-1536 well plate formats) and barcode reader with flexible barcode handling